Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
When the multinucleated osteoclasts were formed, the medium was aspirated, the cells were washed with phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde at room temperature for 20 min, and stained for TRAP using a leukocyte acid phosphatase kit. After the staining, multinucleated osteoclasts which contained 3 or more nuclei, could be identified by counting the number of nuclei in an osteoclast using a Leica light microscope (Leica).
Copyright and License information: ©2019 Song et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
