Culturing of MIN6 Cells

MIN6 is a mouse insulinoma cell line obtained from National Centre for Cell Science (NCCS), Pune, India. MIN6 display many important characteristics that are similar to pancreatic islets (Ishihara et al., 1993). For example, MIN6 cells exhibit glucose-stimulated insulin secretion (GSIS) (Cheng et al., 2012). MIN6 cells were cultured in DMEM supplemented with 10% heat-inactivated FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 2.0 mM glutamine (Purchased from GE Healthcare, Little Chalfont, United Kingdom) in a carbon dioxide incubator maintained at 37°C with 5% CO₂. MIN6 cells with passage number between 5 and 20 were used for all the experiments (Elango et al., 2016).