Cell viability assay (Cell Counting Kit‐8)

Cells were seeded into 96‐well plates, then transfected with siRNA ROR or scrambled negative controls and were cultured for 6, 24, 48, 72 and 96 h. Cell viability was assessed by Cell Counting Kit‐8 assay (Beyotime Institute of Biotechnology, Shanghai, China). The absorbance of each well was read on a microplate reader (F‐2500 florescence spectrophotometer; Hitachi, Tokyo, Japan) at 450 nm. All of the experiments were independently repeated at least three times.