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Cholinesterase inhibition assay

HK Huey Chong Kwong
CK C. S. Chidan Kumar
SM Siau Hui Mah
YM Yew Leng Mah
TC Tze Shyang Chia
CQ Ching Kheng Quah
GL Gin Keat Lim
SC Siddegowda Chandraju
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Ellman’s colorimetric method with minor modification was employed in the determination of AChE and BChE inhibitory activities16. Electric eel acetylcholinesterase (AChE, EC 3.1.1.7), equine serum butyrylcholinesterase (BChE, EC 3.1.1.8), 5,5′-Dithiobis(2-nitrobenzoic acid) (DTNB) and sodium phosphate dibasic (Na2HPO4) were purchased from Sigma-Aldrich (USA). S-butyrylthiocholine chloride (BTCC) from Sigma-Aldrich (Switzerland), acetylthiocholine iodide (ATCI) from Sigma-Aldrich (United Kingdom) and sodium phosphate monobasic (NaH2PO4) from Sigma-Aldrich (Germany) were purchased. Tacrine hydrochloride was purchased from Cayman Chemical Company and absolute ethanol from Merck Emsure® Germany was used as the solvent. Ethanol was used to replace the sample as a control in this assay31. Tacrine hydrochloride was used as the reference drug in the inhibition assays of AChE and BChE.

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