Behavioral Test

We measured the latency from PTZ injection to myoclonic jerks, the first observable behavioral response, and the latency from PTZ injection to generalized tonic-clonic seizures. The behavioral changes were recorded for 30 min (n = 15). The seizure score was evaluated as follows [32, 33]: Score 0, no behavioral seizure; Score 1, myoclonic jerks; Score 2, Straub’s tail; Score 3, clonus. After the behavioral test, the mice were humanely decapitated under inhalation anesthesia for Western blotting and immunofluorescence.