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STO Culture and Actinomycin Treatment

RS Ranjita Sengupta
CM Chandreyee Mukherjee
NS Nandita Sarkar
ZS Zhihong Sun
JL Jacob Lesnik
JH Joseph Huang
BL Biao Lu
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STO cells were cultured in DMEM high-glucose (Life Technologies), supplemented with 2 mMGlutaMax (Life Technologies), and 1 % nonessential amino acid (Life Technologies). Cells were treated with 10 μg/ml actinomycin C (Sigma) for 3 h. The actinomycin C-treated STO non-dividing cells were extensively washed in PBS and plated at 35, 000 cells/cm2 in gelatin-coated tissue culture dish.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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