Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
In a fluorimetric assay,42 increasing numbers of HUVEC-derived MPs were added to human plasma. A thrombin fluorogenic substrate (Z-Gly-Gly-Arg-AMC) and CaCl2 (25 mM) were added to the plasma to initiate the reaction. Thrombin generation, as indicated by fluorescent absorbance, was monitored with an automated coagulation analyzer (Ceveron alpha TGA, Technoclone).
Copyright and License information: ©2018
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
