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Differential scanning calorimetry (DSC) for thermal stability analysis

HC Heather A. Cooke
JA Joe Arndt
CQ Chao Quan
RS Renée I. Shapiro
DW Dingyi Wen
SF Susan Foley
MV Malgorzata M. Vecchi
MP Martin Preyer
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Measurements were made on a VP-DSC Capillary Cell Microcalorimeter (MicroCal Inc.). Proteins were prepared at 1 mg/mL in PBS, pH 7.2. Each sample plus a buffer reference was scanned from 20 to 110°C at 200°C/h with a 5 min prescan hold. The data was analyzed using Origin 7.0 Software. The buffer reference was subtracted from the data, which was next normalized based on concentration. The data was fit using the non-2-state model.

Copyright and License information:  ©2018 The Author(s). Published with license by Taylor & Francis Group, LLC
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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