Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
2.8. siRNA‐mediated STAT3 knockdown
Request a Protocol
Ask a
question
Favorite
Cells were seeded in a six‐well plate. After 24 hours, siRNA duplex targeting STAT3 was transfected using Lipofectamine 2000 (Invitrogen Life Technologies, Los Gatos, CA, USA) according to the instructions. The siRNA‐STAT3 sequence was 5′‐CGTCATTAGCAGAATCTCA‐3′.
Copyright and License information: ©2019 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
