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RuCl3·nH2O, sodium nitrite, triethanolamine, N,N′-dicyclohexylcarbodiimide (DCC), and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich Chemicals (Brazil) as high purity reagents. VDAC 1/2/3, FL-238, and rabbit polyclonal IgG (200 μg mL−1) were acquired from Santa Cruz Biotechnology (Santa Cruz, USA). Doubly distilled water was used in all experiments. Nitric oxide was prepared by the reaction of nitric oxide with copper turnings and was purified according to the literature method [37].
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