PP2A activity assay

RP Ryan M. De Palma
SP Stuart R. Parnham
YL Yitong Li
JO Joshua J. Oaks
YP Yuri K. Peterson
ZS Zdzislaw M. Szulc
BR Braden M. Roth
YX Yongna Xing
BO Besim Ogretmen
request Request a Protocol
ask Ask a question
Favorite

Protein lysate (100 µg) was used for PP2AC immunoprecipitation with a phosphatase kit (MilliporeSigma, Burlington, MA, USA), as described by the manufacturer with the exception of using purified rabbit nonmuscle myosin IIa (MYH9) (Cytoskeleton, Denver, CO, USA) as a substrate.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A