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Immunohistochemistry

MG Manish K. Gupta
JG Jennifer Gordon
GG Gregory M. Glauser
VM Valerie D. Myers
AF Arthur M. Feldman
JC Joseph Y. Cheung
KK Kamel Khalili
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For histopathological examination, hearts were dissected out of mice and fixed in 10% buffered formalin. After fixation, hearts were divided in half, and remaining blood was removed. Fixed heart tissue was processed and blocked in paraffin for sectioning at 5 µm thickness. For staining, tissue sections were deparaffinized in xylene and rehydrated through decreasing-percent alcohols. Sections underwent antigen retrieval in heated citrate buffer, followed by blocking at room temperature for 1 h. Further, slides were stained with respective antibodies and nucleus was stained with DAPI as described earlier. Sections were mounted with coverslip using Vectashield Hard Set mounting medium (Vector Labs) and images were captured under 710 confocal microscope (ZEISS).

Copyright and License information: The Author(s) ©2018
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