Genomic DNA Extraction

Colonies were harvested from LJ slants for genomic DNA extraction, transferred to Tris-HCl–EDTA buffer and heated for 2 hours at 90°C. Genomic DNA (gDNA) was extracted using the cetyltrimethylammonium bromide (CTAB)-phenol chloroform method [21], 2 ul of isolated gDNA was quantified using NanoDrop 2000 spectrtophotometer (Thermo Scientific, USA). 260/280 ratio of all gDNA preps was between 1.7 and 2.0. The gDNA was diluted to 30 ng/ul and 50 ul was shipped to Wadsworth Center, New York State Department of Health, Albany, New York, USA for WGS analysis.