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2.4. Preparation of protein extracts and Western blotting
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Protein extracts were prepared from SH-SY5Y cells as described previously [28]. 40 μg of proteins were electrophoresed, and Western blotting was carried out with a chemiluminescence Western blotting kit as described [28]. The signals were detected by using a Carestream image station 4000MM Pro, and quantitation was performed as described [28].
Copyright and License information: ©2018 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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