The homologous gene pairs among the Gossypium CBLs were searched by the MCScanx software (http://chibba.pgml.uga.edu/mcscan2/). The gene collinearity results were obtained by CIRCOS program (http://www.circos.ca/). The ratio of Ka (nonsynonymous substitution rate) to Ks (synonymous substitution rate) of the CBL genes were estimated by PAML program (http://abacus.gene.ucl.ac.uk/software/paml.html).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.