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To examine the levels of NO and PGE2, chondrocytes were exposed to IL-1β (10 ng/ml) with or without different concentrations (25 and 50 μM) of Schisandrin A. Cell culture supernatants were harvested and stored in −80°C. Griess reaction was performed to measure the NO concentration and PGE2 level was detected with an ELISA kit following the manufacturer’s protocol. All assays were performed in triplicate.
Copyright and License information: ©2019 Tu, Huang, Xiao, Song, Ma, Yan, You and Wu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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