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Cyclooxygenase (COX)-Inhibitory Assay

CL Chiming Lee
JL Jiahnhaur Liao
SC Seuhwa Chen
CY Chiaohan Yen
YL Yuchieh Lee
SH Shihhao Huang
SH Shengtung Huang
CL Chunmao Lin
VC Vincent Hungshu Chang
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A cyclooxygenase (COX) activity assay was performed according to Yang et al. (2001). Inhibitory actions of F-RUT and RUT toward COX-1 and COX-2 activities were individually determined using a fluorometric COX inhibitor screening assay kit as recommended by the manufacturer (catalog no. K548-100 for COX-1, and K547-100 for COX-2, Biovision, Milpitas, CA, United States). The assay directly detects fluorometric PGG2 generated by the COX enzyme at Ex/Em = 535/587 nm using a microplate reader (Thermo Scientific, Waltham, MA, United States). The average fluorescence was calculated for all samples (n = 3) to determine percent inhibition.

Copyright and License information:  ©2019 Lee, Liao, Chen, Yen, Lee, Huang, Huang, Lin and Chang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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