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2.3. Determination of plasma membrane integrity
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Plasma membrane integrity was assessed by flow cytometry using Propidium Iodide (PI, Sigma-Aldrich) staining. PI was added to yeast cell suspensions to a final concentration of 5 μg/mL and incubated for 10 min at room temperature. Cells with red fluorescence [FL-3 channel (488/620 nm)] were considered to exhibit compromised plasma membrane integrity.
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