Amino acid sequences of GlpO from M. capricolum subsp. capripneumoniae ILRI181 and F38T [15], 1601 [17], 9231-Abomsa [18], M. mycoides subsp. mycoides Afadé, B237 [19] PG1 [20], T1/44 [21] and Gladysdale [22], M. leachii PG50T [22], M. capricolum subsp. capricolum ATCC27343T (GenBank accession NC_007633) and M. feriruminatoris G5847T [23, 24] (used as an outgroup) were retrieved from GenBank. Multiple alignments were generated using MUSCLE [25], curated from unreliable sites with Gblocks [26] and the phylogenetic tree was constructed by the Maximum Likelihood method using PhyML [27].
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.