4.2. RNA immunoprecipitation

Immunoprecipitation (IP) and total RNA purification were performed with the RiboCluster Profiler RIP-Assay Kit (MBL, RN1001). HeLa cells transfected with mGFP-tagged expression constructs of ADAR isoforms [³⁷] were washed in PBS(-) five times, and immunoprecipitated with anti-GFP antibody (Clonetech) or normal rabbit IgG as a control. For Western blotting and silver staining, 2 μl of lysate and 100 μl of final wash mixture were used as input and IP samples, respectively. Total RNA was purified from the remaining samples according to the manufacturer’s protocol, and treated with 0.88U TURBOTM DNase (Invitrogen).