Cell culture supernatants were collected from tenocytes stimulated with 1 µg/ml S100A8 & S100A9, 10 ng/ml IL-1ß for 24 hours and the concentrations of IL-6, IL-8, CCL2 (Thermo Fisher Scientific, range 2000-31.25 pg/ml), CCL20, CXCL10 (Biolegend, CCL20 range 160-5 pg/ml, CXCL10 1000-15.6 pg/ml)), S100A8 and S100A9 (R & D systems, range 2000-31.25 pg/ml) were determined using commercially available ELISA kits. Cell culture supernatants were diluted with assay diluent to achieve concentrations within the specified range. Optical density was measured at 450 nm by a microplate reader.
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