Measurement of cytokine release by ELISA

LC Lindsay A. N. Crowe
MM Michael McLean
SK Susan M. Kitson
EM Emma Garcia Melchor
KP Katharina Patommel
HC Hai Man Cao
JR James H. Reilly
WL William J. Leach
BR Brain P. Rooney
SS Simon J. Spencer
MM Michael Mullen
MC Max Chambers
GM George A. C. Murrell
IM Iain B. McInnes
MA Moeed Akbar
NM Neal L. Millar
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Cell culture supernatants were collected from tenocytes stimulated with 1 µg/ml S100A8 & S100A9, 10 ng/ml IL-1ß for 24 hours and the concentrations of IL-6, IL-8, CCL2 (Thermo Fisher Scientific, range 2000-31.25 pg/ml), CCL20, CXCL10 (Biolegend, CCL20 range 160-5 pg/ml, CXCL10 1000-15.6 pg/ml)), S100A8 and S100A9 (R & D systems, range 2000-31.25 pg/ml) were determined using commercially available ELISA kits. Cell culture supernatants were diluted with assay diluent to achieve concentrations within the specified range. Optical density was measured at 450 nm by a microplate reader.

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