Oil Red O stain

Cells were fixed with 10% formalin, rinsed with PBS, then dehydrated with 60% isopropanol for 5 minutes. Cells were subsequently stained with filtered oil red O staining solution for 30 minutes (60% Oil Red O solution [0.5% Oil Red O in isopropanol]/40% water.) Cells were washed twice with PBS, then lipid staining was visualized via microscopy. Following imaging, the dye was extracted with 100% isopropanol and the absorbance was measured spectrophotometrically at 492nm.