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HT-29 cells and HCT-116 cells were cultured in a six-well culture plate for 24 h. Subsequently, wounds were created in the confluent cells using a 10-µl pipette tip. The debris was removed by washing with serum-free medium, and cells were treated with EVO (control or 1.5 μmol/l). After 24 h of incubation, cells that migrated into the wounded area or protruded from the border of the wound were photographed under an inverted microscope. Each experiment was conducted independently three times.
Copyright and License information: ©2019 The Author(s). Published by Wolters Kluwer Health, Inc.
This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/
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