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CELseq2 for bulk samples

JG Jan. P. Gerlach
JB Jessie A. G. van Buggenum
ST Sabine E. J. Tanis
MH Mark Hogeweg
BH Branco M. H. Heuts
MM Mauro J. Muraro
LE Lisa Elze
FR Francesca Rivello
AR Agata Rakszewska
AO Alexander van Oudenaarden
WH Wilhelm T. S. Huck
HS Hendrik G. Stunnenberg
KM Klaas W. Mulder
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Sequencing library generation was performed according to the CELseq2 protocol with minor adaptations, as described13,24. Purified RNA was added into 96 wells plates containing CELseq2 compatible primers. Reverse transcription was performed in 2 μl reactions overlaid with 7 μl Vapor-Lock (Qiagen) using the Maxima H minus reverse transcriptase (ThermoFisher). Further steps were performed as described13. Sequencing was performed using the Nextseq 500 from Illumina.

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