Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Caspase-3 activity assay

LB Louisa Béchohra
FL Fatima Laraba-Djebari
DH Djelila Hammoudi-Triki
ask Ask a question
Favorite

Activity of caspase-3 was performed using CASP-3-C colorimetric assay kit (Sigma-Aldrich, USA), according to the manufacturer’s instructions. Cell lysates obtained from F3 fraction-treated or -untreated NCI-H358 cells were incubated with 2 mM Caspase-3 substrate (Ac-DEVD-pNA) and simultaneously incubated with or without the caspase-3 inhibitor Ac-DEVD-CHO (200 μM) for 1.5 h at 37 °C. Cisplatin was considered the positive control. The enzyme activity was followed by yellow color development at 405 nm and the results were calculated using p-nitroaniline calibration curve.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A