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The 21 polymorphic SSR primer pairs from Pan30 were used in this study based on their high PIC values of greater than 0.787,40,41. All forward primers were labeled with the fluorescence dye, 6-carboxy-fluorescein (FAM). Serials of PCR-cycling conditions were performed to detect the SSR DNA fingerprints7,30. The PCR products for the capillary electrophoresis (CE) were conducted on ABI 3730XL DNA Analyzer (Applied Biosystems Inc., Foster City, CA, USA) following the manufacturer’s instructions to generate GeneScan files.
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