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Protein lysate was extracted from MCF-7 and MDA-MB-231 cells and the total protein of cell lysates was determined using Bradford method. In proteome analysis, the targeted protein was identified using Proteome Profiles Human Apoptosis Array kit (R&D systems). The experiment was carried out according to the instruction provided by the manufacturer. The protein arrays on the membranes were scanned using ChemiDoc MP Imaging System (BIO-RAD, USA). The data were analyzed using Image Lab 4.1 software.
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