Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

6.3. Dynamic Light Scattering (DLS)

HL Hsing-Ying Lin
CC Chung-Yu Chou
NS Noah Sadik
request Request a Protocol
ask Ask a question
Favorite

DLS is a technique that reflects scattering light intensity distribution under Brownian motion of suspended particles within a time period [50]. It can provide information of mean particle size and polydispersity index (PDI) [51]. It has been used to characterize the size of EVs derived from cells [52,99,100]. The diameter of the EVs is obtained from application of the Stokes–Einstein equation. However, in polydispersed suspensions, larger particles (e.g., microvesicles) generate more scattering light than smaller EVs, resulting in the inaccuracy for EV analysis and a bias toward the detection of larger particles. Additionally, it is unable to provide any biochemical information about cellular origin of EVs.

Copyright and License information:  ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A