Hematoxylin and eosin (H&E) staining

Testicular morphology was examined using the H&E method. The tissue slides were deparaffinized in xylene and rehydrated in gradual concentrations of ethanol for 5 min. Then, they were incubated in Harris hematoxylin solution for 10 min at room temperature and rinsed in running water for 10 min before incubation in eosin solution for 20 min at room temperature. Finally, they were dehydrated in gradual concentrations of ethanol for 3 min, cleared in xylene for 5 min, and mounted. The nucleus was visualized in purple color, and the cytoplasms were visualized in red color. The stained tissue slides were assessed using the light microscope with Optilab^® camera. The morphology and structure of the spermatogenic cells in the seminiferous tubules were observed and analyzed descriptively.