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Angiotensin II (Ang II) induced cardiomyocyte hypertrophy
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H9c2 rat cardiomyocytes were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with 10% foetal bovine serum under 5% CO2 at 37 °C. After 12 hrs of serum starvation, H9c2 cardiomyocytes were stimulated with 1 µM angiotensin II (Ang-II) (Sigma Aldrich) or 7.5 ng/ml transforming growth factor-β (TGF-β) (Peprotech) for 24 hrs, 48 hrs and 72 hrs. Expression of hypertrophic genes atrial natriuretic protein (ANP) was measured at mRNA and protein levels to confirm the development of hypertrophy.
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