2.9. Pharmacokinetic Studies in BALB/c mice

To determine dose-dependent serum drug concentrations, the in vivo pharmacokinetics of free Cmpd I and Cmpd I-NP were investigated on BALB/c mice obtained from Jackson Laboratory. BALB/c mice were randomly divided into two groups containing 3 mice each. One group of mice was injected intraperitoneally (i.p.) with a single 100 mg/kg dose of free Cmpd I suspended in sterile saline solution containing 10 % Tween 80. The second group was injected intraperitoneally with single 125 mg/kg Cmpd I-NP dose suspended in sterile saline solution. The blood samples were collected at predetermined time points from the ocular venous plexus by retro-orbital venipuncture and used for subsequent analysis as detailed previously.