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Single cell suspensions from lymph nodes of DO11/Rag2−/−/CD90.1+ (i.e., DO11 T cells) mice were prepared and 2×106 LN cells adoptively transferred intravenously into gender-matched K5/TGO/TCRα−/− recipient mice, and mice were started on doxycycline chow the same day as adoptive transfer.
Copyright and License information: ©2018
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