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Hepatic lipid analysis

SG Scott M. Gordon
EN Edward B. Neufeld
ZY Zhihong Yang
MP Milton Pryor
LF Lita A. Freeman
XF Xiao Fan
IK Iftikhar J. Kullo
LB Leslie G. Biesecker
AR Alan T. Remaley
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Liver tissue harvested from PBS perfused mice was snap frozen with liquid nitrogen and stored at −80 °C until lipid extraction. A portion of liver (80–100 mg) from the lateral left lobe was used for lipid extraction. Tissue was added to 1 mL 0.9% NaCl, minced and lipids collected by chloroform methanol extraction. Chloroform layer containing lipid was dried under nitrogen gas. Dry lipid was solubilized in hexane isopropanol (3:2, v:v) and a small volume used for measurement of cholesterol, triglyceride, and free fatty acids by enzymatic assay. All measurements were adjusted to the starting liver tissue mass.

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