Injections of L-741,626

To detect whether dopamine neurotransmission was necessary for the observed behavioral effects in males, four of them were injected with L-741,626 (Sigma-Aldrich, Saint Louis, MO, USA), a very selective antagonist of D2-receptors, which had been used to study the function of D2-receptors in rodents (Dai et al., 2016; Li et al., 2010; Watson et al., 2012) and primates (Achat-Mendes et al., 2010). We injected L-741,626 intraperitoneally at 3.33 µg/g body weight, within the range described for rodents (Li et al., 2010; Watson et al., 2012), diluted in saline (acetic acid was added to increase solubility at first, then pH was neutralized by caustic soda solution). The L-741,626 injections were administered 30 min before each test with at least 48 hr between treatments, 5 times for each animal, with an intra-individual control of sham injections (saline) of the same volume.