On day 28 following MCT exposure, following haemodynamic measurements, the hearts of all 40 rats were collected and right ventricular hypertrophy was measured by Fulton’s index as previously described.23 Hearts were isolated, flushed with 0.154 M saline, and dissected to separate the right ventricle from the left ventricle plus septum. As indices of right ventricle hypertrophy, the ratios of right ventricle weight/left ventricle plus septum weight were determined.
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