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Quantifying Pseudonodules

CG Christopher Gauthier-Coles
RW Rosemary G. White
UM Ulrike Mathesius
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Plate-grown plants were harvested 4 weeks following treatments; plants grown in vermiculite were harvested 3 weeks after the first treatment and gently rinsed in tap water. Pseudonodules were counted with the aid of a magnifying glass or stereo microscope. Pseudonodules were identified by their bulbous shape, which contrasted with emerging lateral roots identified by their clearly recognizable conical shape.

A Chi-square analysis was performed between each cytokinin treatment and control to determine the statistical significance of the number of replicates producing pseudonodules using Prism from GraphPad (La Jolla, CA, United States). A non-parametric Kruskal–Wallis test with a Dunn post hoc test was performed between treatments producing pseudonodules in order to determine cytokinin concentration-dependent frequency of pseudonodulation.

Copyright and License information:  ©2019 Gauthier-Coles, White and Mathesius.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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