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In silico Evaluation for Microsatellite Transferability Across Species

RD Ritwika Das
VA Vasu Arora
SJ Sarika Jaiswal
MI MA Iquebal
UA UB Angadi
SF Samar Fatma
RS Rakesh Singh
SS Sandip Shil
AR Anil Rai
DK Dinesh Kumar
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In order to evaluate this module of PolyMorphPredict, public domain data was used. Available sweet cherry (Prunus avium) genome was used as focal species and peach (Prunus persica) was used as host species. A total of 200 microsatellite markers having simple dinucleotide repeats from chromosome 1 of cherry genome assembly were downloaded1. e-PCR was carried out using whole genome sequence of Prunus as template. True e-PCR amplicons of microsatellite loci were obtained by filtering out amplicon size of >500 bp and asymmetric match with either forward or reverse primer sequence.

Copyright and License information:  ©2019 Das, Arora, Jaiswal, Iquebal, Angadi, Fatma, Singh, Shil, Rai and Kumar.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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