Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Real-time Reverse-Transcription Quantitative PCR (RT-qPCR)
Request a Protocol
Ask a
question
Favorite
Total RNA was extracted using TRIzol Reagent (Thermo Fisher Scientific) per instructions in the manual. cDNA was synthesized using QuantiTect Reverse Transcription Kit (QIAGEN). PCR reactions were prepared using QuantiFast SYBR Green PCR Kit (QIAGEN). Real-time RT-PCR was performed using an Applied Biosystems StepOnePlus Real-Time PCR System in a two-step cycling protocol, with a denaturation step at 95 °C and a combined annealing/extension step at 60 °C. The primers (S1C Table) were purchased from Integrated DNA Technologies.
Copyright and License information: ©2019 Xi et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
