Cell viability assay

The effect of MOEO and Oseltamivir on the viability of MDCK was determined by 3-[4,5-dimethyl thiazol-2-yl]-2,5-diphenyl tetra zolium bromide (MTT) assay. MDCK cells were grown (1 × 10⁴ cells/well) in 96-well plate for 24 h. The medium was replaced with DMEM containing different concentration of component (MOEO and oseltamivir). Then the cells were further incubated for 48 h. After incubating the cells for specific time at 37 °C, MTT (5 mg/ml in PBS) was added to each well and the cells were incubated for 2 h. After removal of supernatant, 50 µl of DMSO was added and incubated for 30 min. Next, the absorbance was recorded on a micro plate reader at the wave length of 540 nm [14].