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Measurement of pro-inflammatory cytokines and chemokine
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Pro-inflammatory cytokines interleukin-6 (IL-6) and IL-1β in plasma and lung tissues were quantified by using the mouse ELISA kits (BD Biosciences, Franklin Lakes, NJ). Macrophage inflammatory protein (MIP)-2 in plasma and lung tissues was measured by using the mouse ELISA kit (R&D Systems).
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