Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Activated partial thromboplastin time (aPTT) assay.

UW Ursula Waack
MW Mark Warnock
AY Andrew Yee
ZH Zachary Huttinger
SS Sara Smith
AK Ayush Kumar
AD Alban Deroux
DG David Ginsburg
HM Harry L. T. Mobley
DL Daniel A. Lawrence
MS Maria Sandkvist
request Request a Protocol
ask Ask a question
Favorite

Concentrated cell-free supernatants were mixed with normal (healthy) human pooled plasma samples (George King) for 20 min at 37°C. An equal volume of aPTT reagent (Pacific Hemostasis) was added. After incubation for 5 min, CaCl2 (final 10 mM) was added. Clot times were determined using an Amelung KC4A Micro Coagulation analyzer.

To identify the target of CpaA, a modified aPTT assay was performed by first incubating concentrated AB031 supernatant with normal plasma. After incubation, the plasma was diluted 1:100 and added to human plasma samples derived from donors deficient in congenital coagulation factor (George King), and an aPTT assay was performed as described above.

Copyright and License information:  ©2018 Waack et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A