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The vanadate-sensitive ATPase activity of ABCB1 and ABCG2 using crude membranes of High Five insect cells was determined with the presence of DJ95 (0–10,000 nM) or positive substrate drug by PREDEASY ATPase Kits with modified protocols as previously described (Ambudkar, 1998; Wang et al., 2017b). Paclitaxel and topotecan were selected as positive substrate drugs for ABCB1 and ABCG2, respectively.
Copyright and License information: ©2019 by The American Society for Pharmacology and Experimental Therapeutics
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