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2.12. Flow cytometry analysis of the cell cycle
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The cells were cultured in serum‐free medium for 24 hours and then cultured in medium with 10% FBS for 24 hours. The indicated cancer cells were collected and fixed with precooling 75% ethanol at 4°C. The following day, the 75% ethanol was removed, and the cells were incubated in propidium iodide solution (100 μg/mL) at room temperature for 20 minutes in the dark. The cell cycle experiment was analyzed by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).
Copyright and License information: ©2019 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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