Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

GFR

KN Kristina M. Niculovic
LB Linda Blume
HW Henri Wedekind
EK Elina Kats
IA Iris Albers
SG Stephanie Groos
MA Markus Abeln
JS Jessica Schmitz
EB Esther Beuke
JB Jan H. Bräsen
AM Anette Melk
MS Mario Schiffer
BW Birgit Weinhold
AM Anja K. Münster-Kühnel
request Request a Protocol
ask Ask a question
Favorite

The GFR of control and PCmas−/− mice was determined by transcutaneous measurement of FITC-sinistrin as described previously.21 Briefly, fluorescent signals were measured with a Transdermal GFR Monitor (MediBeacon GmbH, Mannheim, Germany) before and after retrobulbar injection of FITC-sinistrin (7.5 mg/100 g body wt) in anesthetized mice. The GFR was calculated from the t1/2 of FITC-sinistrin in the blood.

Copyright and License information:  ©2019 by the American Society of Nephrology

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A