Mouse model of cisplatin nephrotoxicity

Male C57BL/6 mice (8 wk of age) purchased from Hunan Slack King Experimental Animal company (Changsha, China) were injected intraperitoneally with a single dose of 30 mg/kg body weight cisplatin. Control mice were injected with a comparable volume of saline. To study the effects of FGF21, recombinant FGF21 was dissolved in PBS and administered intraperitoneally at 0.1 μg/g body weight 1 h before cisplatin injection followed by injection every 8 h. A comparable volume of PBS was given to the no-FGF21 animals. Mice were euthanized at d 3. All animal experiments were performed according to a protocol approved by the Institutional Committee for the Care and Use of Laboratory Animals of Second Xiangya Hospital at Central South University.