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The GALT enzyme activity was detected by an enzymatic-fluorometric method [29]. The fluorescence reading at 460 nm was obtained with a Hitachi F-2000 fluorometer (Hitachi, Tokyo, Japan) and to measure haemoglobin concentration, an absorbance reading at 410 nm was obtained with a Hitachi U-2001 spectrophotometer (Hitachi, Tokyo, Japan). The normal range was defined as 37– 66 μmol/h per gHb.
Copyright and License information: Garcia et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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