The dynamic light-scattering measurements were performed at 25 °C by using a Viscotek model 802 dynamic light-scattering instrument (Viscotek Europe Ltd.) as previously reported (Li et al., 2009). The hydrodynamic radius RH was calculated with the regularization histogram method by using the spheres model, from which an apparent molecule mass was estimated according to a standard curve calibrated form known globular protein. OmniSIZE 2.0 software was used to calculate the size distribution of aggregated protein from the addition of EGCG.
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