Liposome aggregation was assessed by Dynamic Light Scattering (DLS). A unique population of LUVs DOPC, DOPG or DOPC/DOPG was incubated at 37 °C with 5 μM of each protein/peptide for 15 min. Particle size distribution was measured with a Zetasizer Nano ZS (Malvern Instruments Ltd., Worcestershire, UK). Polydispersity of LUV population was also analyzed. Size radius was plotted versus protein concentration.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.