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LC-MS method for measurement of intact-antibody mass
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LC-MS measurements of the mass of intact antibodies were acquired by using an ultra–high-performance LC system and a Synapt G2 mass spectrometer (Waters, Milford, MA). A PLRP-S column (4,000 Å, 8 µm, 150 × 2.1 mm; Agilent, Santa Clara, CA) was used for reverse-phase separation. Mobile phases A and B were 0.05% TFA in water and acetonitrile, respectively. Mass spectra were collected at a mass-to-charge ratio range of 800–4,500. Molecular mass was determined by deconvolution of the mass spectra data, using the MaxEnt I software package.
Copyright and License information: ©2018 The Author(s). Published by Taylor & Francis
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
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