2.6 Complementarity Determining and Framework Region Mutation Analysis

Nucleotide substitution mutation data for complementarity determining and framework regions for IgH and Igκ were obtained from the IMGT HighV-Quest tool. Any mutations involving degenerate bases were removed. Nucleotide range (in base pairs), number of reads containing at least one mutation, total number of substitution mutations, and number of mutations per base pair position were determined for each region. Comparative values for each combination of region, Ig location, and treatment group were determined by calculating the average of values contained in each combination’s respective replicates (n=3).